Cristiano Ronaldo limped out of Portugal training again on Wednesday amid concerns that his World Cup participation could be in doubt.
The Real Madrid forward, 29, left the field early with an ice pack strapped to his left knee.
The world player of the year has suffered from knee and thigh injuries since the end of the domestic season.
Portugal, who lost their opening Group D match 4-0 to Germany, face USA on Sunday.
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